Briefly describe the use method and purchase requirements of the electrophoresis instrument

Electrophoresis instrument: Electrophoresis technology is an indispensable and important analytical method for molecular biology research. Electrophoresis is divided into two categories: free interface electrophoresis and zone electrophoresis:

1. Free interface electrophoresis does not require supports, such as isoelectric focusing electrophoresis, isokinetic electrophoresis, density gradient electrophoresis, and microelectrophoresis. This type of electrophoresis has been rarely used at present.

2. Zone electrophoresis requires the use of various types of substances as supports. Commonly used supports include filter paper, cellulose acetate film, non-gel support, gel support and silica gel-G thin layer, etc. The most commonly used in the field of science is agarose gel electrophoresis.

The following is a brief introduction to its use:
1. First connect the two electrodes of the electrophoresis tank to the DC output terminal of the electrophoresis instrument with a wire, and pay attention not to reverse the polarity.
2. The power switch of the electrophoresis instrument is turned to the off position, the voltage knob is turned to the minimum, and the voltage stabilizing current mode and the voltage and current range are selected according to the work needs.
3. Turn on the power, slowly rotate the voltage adjustment knob until the desired voltage is reached, and set the electrophoresis termination time, at which time electrophoresis begins.
4. After the work is completed, the knobs and switches should be turned to zero or off, and the electrophoresis plug should be pulled out.

【Shopping Reference】

According to the experimental requirements, the electrophoresis instrument with the corresponding specifications can be selected to ensure economical, durable, simple and reasonable. Because the internal structure characteristics of high and low voltage electrophoresis instruments are different, because high-pressure electrophoresis instruments often have multiple functions attached, and high-pressure electrophoresis instruments must not operate without load (easy to cause personal injury and damage to internal devices), resulting in different degrees of difficulty Wait. Therefore, the correct purchase method is: "According to the highest voltage required by the experiment, determine the specification and model of the electrophoresis instrument to be purchased."