How to identify fake and inferior ELISA kits

With the rapid development of life science and technology, biological companies have sprung up like mushrooms, and various types of biological reagent products have greatly facilitated the work of scientific researchers. Undoubtedly, a healthy and orderly biological reagent industry environment and order play a decisive role in the healthy development of the entire industry. However, recently, many customers have reported to us that some profit-making companies and small workshops have used fake and inferior ELISA kits to harm the majority of scientific researchers. They often profit-only, ignoring the rigor and seriousness of scientific research, and let the majority of scientific researchers Passive counterfeiting seriously disrupts scientific research and market order, so we have an obligation and responsibility to disclose the methods used by these counterfeiters to make counterfeit and inferior ELISA kits, and to provide methods for identifying these counterfeit and inferior ELISA kits for The majority of scientific researchers and users refer to those who hang sheep's heads to sell dog meat, import counterfeit reagents, and have no integrity, forgery, such as rats crossing the street, everyone shouts.

Fake one: register a company abroad, produce domestically, conceal the place of production, obviously it is a domestic reagent, but claim to falsely report high prices for foreign imports, deceiving the majority of users.

Identification method: search the keywords "manufacturer name", "ELISA" and "fake" on Google, and users of various forums will discuss the quality and effectiveness of the ELISA kit one by one.

Fraud II: counterfeiting imported ELISA kits makes the quality of imported sub-packaged ELISAs uneven, mixed with fish and dragons, many of them are processed by small workshops, such kits can occasionally be lucky to make the result, if they ca n’t do it, the purchased Of companies do not have perfect after-sales services, often pushing customers' problems and shirk their responsibility, and ultimately consumers suffer.

Identification method: directly call the manufacturer, ask them for the source of the kit antigen and antibody, ask for the kit instructions, and then use the Internet to confirm the information. The formal ELISA manufacturer, the information is very complete, if the information is incomplete, then The manufacturing level and quality of the ELISA will be very poor.

Counterfeiting 3: In order to exaggerate the range of indicators covered by their production ELISA, some profiteers use one indicator to impersonate other indicators, causing the illusion that all kinds of species and various indicators can be done. For example, using indicators such as TGFβ to replace other indicators of most kits as detection antibodies can also obtain a good standard curve. Because TGFβ is widely expressed in most mammals (humans, rats, mice, pigs, cattle ...), the amino acid sequence homology of antigens between species reaches 100%, and TGFβ is affected by other factors The expression level will also change accordingly, which is difficult for ordinary customers to detect. Therefore, such vicious frauds cause consumers to passively falsify, and the consequences are extremely serious. Therefore, customers are kindly reminded to be alert to such fraud kits!

Identification method:

â… . The interference experiment can be used to distinguish whether the ELISA kit detects the target antigen. The methods are as follows:

(1) Configure the recombinant protein antigen specific to the kit and the detection antibody in the kit into a mixed incubation solution of antibody: antigen≈1: 2

(2) After incubating at 37 ° C for 15 minutes, replace the detection antibody in the original kit

(3) Follow up the operation according to the instructions of the kit, add the detection antibody, enzyme complex and substrate

(4) After the experiment is completed, the standard curve is tested. If the standard curve is good, it indicates that the added target antigen and the kit antibody do not match. The detection antibody of the kit is for a non-target antigen. This kit is a fake and fake ELISA kit.

(5) If the standard curve is wireless, it means that the detection antibody of the kit has been neutralized or interfered with the target antigen, which affects the detection of the actual kit antibody. The detection antibody of the kit is directed to the target antigen.

â…¡. If two ELISA kits A and B of the same company are purchased, cross-experiment can be used to distinguish whether the ELISA kit is falsified. The method is as follows:

(1) Take out the two coated plates of the purchased kit A.

(2) A standard of coating plate plus kit A is used as the standard curve.

(3) Another standard coated plate plus kit B is used as the standard curve.

(4) Follow-up operation is carried out according to the instructions of the kit, adding detection antibody, enzyme complex and substrate.

(5) After the experiment is completed, two standard curves are detected. If the two standard curves are consistent, it means that the two ELISA kits detect the same index instead of A and B, that is, the kit is a fake and fake ELISA kit.

(6) If the two standard curves are inconsistent, it means that the two indicators of the two ELISA kits are different. Kit A can only detect A, but not B, that is, the kit is a normal ELISA kit.