English name: Acridine orange; Basic orange 3RN; Rhoduline orange; Euchrysine 3RXA; CI46005
Other names: acridine orange; basic orange 14; 3,6-bis (dimethylamino) acridine zinc chloride hydrochloride
CAS number: 10127-02-3
C17H20ClN3 · 0.5ZnCl2 · HCl = 369.96
Level: Electrophoresis Grade
Spectroscopic effective content: ≥90.0%
Em (490nm, water):> 36500
Properties: orange-red powder. Soluble in water, ethanol shows orange yellow with green fluorescence. In case of concentrated sulfuric acid, there is a colorless green fluorescence, which is orange yellow after dilution. The aqueous solution plus sodium hydroxide produced a yellow precipitate. 1% solution, pH is 6.5, pH value is 8.4 to 10.4, no fluorescence Can be embedded between two adjacent base pairs to loosen part of the double helix DNA, the distance between the two base pairs is doubled. In this process of DNA replication, the DNA strand will be increased or deleted by one base, resulting in a frameshift mutation. Use: Biochemical research. Tumor cells and bacteria, nucleic acid stains, and mutagenic agents for frameshift mutations. Fluorescent indicator. Acridine orange is a fluorescent pigment. The wavelength of the acridine orange excitation filter is 488 nm. The blocking filter wavelength is 515nm. Acridine orange has a difference in the amount of DNA and RNA binding in the cell, which can emit different colors of fluorescence (ie, coloring specificity)
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